KuTenk 2000 - SBIO225404-Cloning, Gene Expression, and Protein Purification—Experimental Procedures and Process Rationale

by Charles Hardin et al. 

2001 (435 pages)

ISBN:9780195132946

Containing 20 experiments and detailed process rationales, this manual will help students prepare, manipulate, and analyzse plasmids, produce fusion proteins in bacteria, and purify proteins based on unique chemical properties or substrate affinities.

Cloning, Gene Expression, and Protein Purification—Experimental Procedures and Process Rationale

Preface

Introductory Unit—Introduction to the Biochemical Laboratory

Part 1 - Nucleic Acids and Cloning

Unit 1

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DNA Isolation

Unit 2

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Construction of Recombinant Plasmids

Unit 3

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The Polymerase Chain Reaction

Unit 4

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Transcription of Genomic DNA and Analysis of the Resulting mRNAS

Unit 5

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Transformation and Gene Expression

Unit 6

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Analysis of DNA or RNA by Duplex Hybridization: DNA Isolation, Labeling, and Probing

Part 2 - Protein Purification

Unit 7

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Protein Purification

Unit 8

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Discontinuous Gel Electrophoresis, Protein Mobilities, and Apparent Size Determination

Unit 9

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Immunochemical Techniques

Unit 10

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Combinatorial Biochemical Technology

Appendix A

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Appendices